Protein release fromEscherichia coli cells permeabilized with guanidine-HCl and Triton X100
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منابع مشابه
Protein release from Escherichia coli cells permeabilized with guanidine-HCl and Triton X100.
An important factor complicating the recovery of recombinant proteins from Escherichia coli is their intracellular location. An alternative to the commonly used method of releasing these proteins by mechanical disruption is to chemically permeabilize the cells. The objective of this research was to characterize the protein release kinetics of a permeabilization process using guanidine-HCl and T...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 1989
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.260330712